Figure 5

C57Bl/6 mouse lung tissue respiration and caspase activity. Panels A–B, in 2 independent experiments, lung fragments were incubated at 37°C in 50 mL KH buffer gassed for 60 sec with 95% O₂ : 5% CO₂ every h. At indicated time periods, samples were removed from the incubation medium and processed for O₂ consumption and caspase activity. The values of k _c (Panel A) and AMC peak area (Panel B) are plotted as a function of incubation time. Panel C–D, respiration of lung fragments that were incubated at 37°C in 50 mL MEM continuously gassed for 60 sec with 95% O₂:5% CO₂.