Exon | Forward | Reverse | Size of fragment | Annealing temp. Sanger sequencing | Annealing temp. HRM |
---|---|---|---|---|---|
2 | GCAATGAAAGAAACACTGGATG | TCAAATCCAATTACCCAGCAG | 358 | [1] | 64 |
3 | TTTTGTAAATTTATTGGAGGACG | TCTGCCAGAAGAGATTAAATGAG | 429 | [1] | 64 |
4 | TTTTGGAGGATAACCTTGGAAC | AAATCAGACAACCAAGGCTACAG | 390 | [1] | 66 |
5 | TTGTCGTCAAGACATGCTTTC | TTTAATATTCATTCATTCATACACTGC | 518 | [1] | 66 |
6 | TAGTGGCATTTCCACCAAAC | GAATTCTTACTCTTGCCCATTCC | 437 | [1] | 69 |
7 | TTCTCACTACTGCAGTATTGATACCTT | GAGCCGCATTAAGCATATTTTC | 303 | [1] (7 F modified) | 69 |
8 | AAATCCCAGTTAAACCCCAC | TCACATCACAACACAGGACG | 554 | [1] | 68 |
9 | TAAATCACCGTCGCCAAATC | AAATCACTCTACAGCTACCTCATGG | 591 | [1] | 69 |
10 | CATGTGTGTCAGAAATGACAGTTG | TTCCTATGGATACAAAGAATAACAAAG | 431 | [1] | 68 |
11 | AGCAGGTTACTTTGGAGTACTGAAG | AACTTTTATTGCTGCACGATACTG | 498 | [1] | 69 |
12 | TCACATTTCAAGTGCTCACC | TGAACATGTAGATGACTACAAAAGC | 596 | [1] | 69 |
13 | TTTTACTAGGCAGGACTTTTAAAGATG | AAGTGTTCATGGTGCATGATTC | 585 | [1] | NA |
14 | TTTGCAGTCCAGCTCATATTG | AAGCTGTGAATCGGAGAAAG | 498 | [1] | 69 |
15 | TAAGAAGTGTCATGCCTCGG | TCTAGTGGAGAAATAGAAGAGGCAC | 468 | [1] | 68 |
16 | GAAAGCATCATTTCTGTTCTGAAG | AAGAGAAAAACGACTCTTTAGC | 443 | 16R is new | 65 |
17 | TTCAGCATACTGTGTTCTACCTCTT | TTTTAGTAGAGACGAGGTTTCACC | 484 | 17 F is new | 69 |
18 | TGTAAAGGTGCCATTTAGCTTC | TTTGTGTGCAAAGCATCTCC | 589 | [1] | 69 |
19 | ATTGCACTTGAGGGATTCTTACC | TTTGTGATATATTAATGGGCCAAG | 496 | [1] | 67 |
20 | TTGGCCCATTAATATATCACA | TCTCACTCCAACTGTTATGGCTTA | 594 | [1] | NA |
21-1 | AATTGCTGTTGCTCTCAGCC | GAGTACATTCATCGCTGGGC | 508 | [1] | 68 |
21-2 | ACAAGCAGGAAATACCCGTG | ACTGCAAACCACTTTCAGGC | 501 | [1] | 68 |
22 | AAAGCATAGAATATGTGGGAATT | AGAAATTTGCCTCCATCAAA | 584 | [1] | NA |
23-1 | AACCCTTGCTTTTATTGAGTTTC | CAGGGCTTCCACACAGTCC | 574 | [1] | NA |
23-2 | AAACTGTGGTGTTGACACGG | TACAAGGCCAACACGATGAG | 571 | [1] | 68 |
24 | TGTGGGGATAGTGTAAATGCTTC | TGCCGTCAGAACTCTGAAAC | 403 | [1] | 68 |
25-26 | TGGACTGCCTGTAAAAGTGG | TGAACTTTTCCCCTTTGATG | 450 | [1] | 66 |
27 | CCTGTCTGTCGGGGGTATG | TCTGCCTTCAATTCATTCCA | 448 | [1] | 69 |