Figure 2From: Identifying disruptors of male germ cell development by small molecule screening in ex vivo gonad culturesExamples of gating strategy for flow cytometric analysis of fetal germ cells and gonadal somatic cells based on a PBS treated control sample. A. Side vs forward scatter plot showing gate P1 around cells and excluding cell debris. B. Propidium iodide in the PE-Cy5 width vs propidium iodide in the PE-Cy5 area showing events from P1, with single cells gated within P2. C. MVH-alexa fluor 488 vs propidium iodide area showing events from P2, with germ cells (MVH positive) gated in P3 and somatic (MVH negative) cells gated in P4. D. EdU-Click-iT 647 in the APC-area channel vs propidium iodide area showing events from P3 (ie germ cells), with proliferating (EdU positive) cells gated in P5. E. EdU-Click-iT 647 in the APC-area channel vs propidium iodide area showing events from P4 (ie somatic cells), with proliferating (EdU positive) cells gated in P6.Back to article page