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Figure 3 | BMC Research Notes

Figure 3

From: A novel pair of inducible expression vectors for use in Methylobacterium extorquens

Figure 3

Single-cell dynamics of P R/cmtO and P R/tetO activation. Histograms of relative fluorescence values for pJP18T (A) and pJP22T (B) harboring M. extorquens PA1 as determined by single-cell flow cytometry. Cultures were grown to mid-log phase and induced with 5 µg/ml Q (A) or 25 ng/ml aTc (B). At times 0, 2, 4, 6, 8, and 24 hrs, cells were harvested and fixed in carbon-free Hypho medium supplemented with 100 mg/ml streptomycin. The 8 and 24 hr time points have nearly overlapping fluorescence distributions. Fluorescence units are presented as arbitrary units (A.U.) and normalized as described in Materials and Methods.

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