Figure 2From: Validation of reference genes aiming accurate normalization of qPCR data in soybean upon nematode parasitism and insect attackPairwise variation (V) analysis of the candidate reference genes as predicted by geNorm . The Pairwise variation (Vn/Vn+1) was analyzed using the normalization factors NFn and NFn+1 to determine the optimal number of reference genes required for effective normalization of qPCR data.Back to article page