Table 1 Primer sequences and amplicon characteristics of tested genes
Gene symbol | Forward primer sequence (5’- 3’) | Reverse primer sequence (5’- 3’) | Amplicon length (bp) | Primer location* | Efficiency (%) |
|---|---|---|---|---|---|
GmCYP2 | CGGGACCAGTGTGCTTCTTCA | CCCCTCCACTACAAAGGCTCG | 154 | S | 98,3 |
GmELF1A | GACCTTCTTCGTTTCTCGCA | CGAACCTCTCAATCACACGC | 195 | D | 102,4 |
GmTUA5 | AGGTCGGAAACTCCTGCTGG | AAGGTGTTGAAGGCGTCGTG | 159 | S | 101,7 |
GmELF1B | GTTGAAAAGCCAGGGGACA | TCTTACCCCTTGAGCGTGG | 118 | D | 92,5 |
GmACT11 | CGGTGGTTCTATCTTGGCATC | GTCTTTCGCTTCAATAACCCTA | 142 | D | 104,1 |
GmUBC2 | TCCCCTCACACCCTTCCTC | CCATCCCAAGGGGTGTCAT | 155 | D | 107,6 |
GmTUB | CCTCGTTCGAATTCGCTTTTTG | CAACTGTCTTGTCGCTTGGCAT | 161 | S | 96,4 |
GmG6PD | ACTCCTTGATACCGTTGTCCAT | GTTTGTTATCCGCCTACAGCCT | 126 | D | 110,7 |
GmUBC4 | GAGCGAGCAGTTTCAGAC | CATAGGAGGGACGATACG | 168 | D | 98 |
GmRB7 | TTGTAGGTGTCTCCGTCGC | AATGCTCTTGGCGGTGATG | 179 | S | 87,3 |