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Table 2 Expression stability values and rankings of the reference genes calculated by NormFinder software

From: Validation of reference genes aiming accurate normalization of qPCR data in soybean upon nematode parasitism and insect attack

A

         

Organs V4

 

Organs R2

 

Organs R4

 

Root - development

Stem - development

Ranking

Stabilityvalue

Ranking

Stability value

Ranking

Stability value

Ranking

Stability value

Ranking

Stability value

GmELF1A

0,189

GmACT11

0,187

GmCYP2

0,169

GmELF1A

0,189

GmCYP2

0,335

GmUBC2

0,321

GmTUA5

0,451

GmELF1A

0,180

GmUBC2

0,190

GmTUA5

0,386

GmACT11

0,429

GmELF1A

0,499

GmACT11

0,188

GmCYP2

0,306

GmUBC2

0,430

GmUBC4

0,535

GmG6PD

0,549

GmTUA5

0,217

GmUBC4

0,331

GmACT11

0,490

GmTUA5

0,593

GmUBC4

0,617

GmUBC4

0,385

GmTUA5

0,390

GmELF1A

0,672

GmELF1B

0,605

GmELF1B

0,692

GmELF1B

0,396

GmELF1B

0,557

GmG6PD

0,767

GmG6PD

0,896

GmUBC2

0,703

GmUBC2

0,450

GmG6PD

0,742

GmUBC4

0,792

GmCYP2

1,046

GmCYP2

0,738

GmG6PD

0,980

GmACT11

0,899

GmELF1B

0,932

GmTUB

2,966

GmTUB

4,138

GmTUB

2,095

GmTUB

1,393

GmTUB

3,529

Best combination

Stability value

Best combination

Stability value

Best combination

Stability value

Best combination

Stability value

Best combination

Stability value

GmELF1A

0,203

GmACT11

0,250

GmCYP2

0,130

GmELF1A

0,167

GmCYP2

0,259

and GmUBC2

 

and GmTUA5

 

and GmELF1A

 

and GmUBC2

 

and GmTUA5

 

B

         

Leaf - development

Spatial/Temporal

M. incognita -inoculated root

A. gemmatalis -infested leaf

Total

Ranking

Stability value

Ranking

Stability value

Ranking

Stability value

Ranking

Stability value

Ranking

Stability value

GmELF1A

0,183

GmACT11

0,298

GmELF1A

0,1333

GmCYP2

0,060

GmACT11

0,089

GmACT11

0,230

GmTUA5

0,349

GmACT11

0,1660

GmELF1A

0,067

GmELF1A

0,124

GmUBC2

0,412

GmUBC2

0,448

GmTUA5

0,2221

GmACT11

0,081

GmTUA5

0,236

GmCYP2

0,433

GmELF1A

0,493

GmG6PD

0,3143

GmG6PD

0,087

GmG6PD

0,348

GmELF1B

0,451

GmG6PD

0,527

GmUBC2

0,3177

GmELF1B

0,121

GmELF1B

0,425

GmTUA5

0,545

GmCYP2

0,669

GmTUB

0,3267

GmTUA5

0,145

GmCYP2

0,559

GmUBC4

0,670

GmELF1B

0,722

GmCYP2

0,3951

GmUBC4

0,222

GmUBC4

0,621

GmG6PD

0,925

GmUBC4

0,748

GmELF1B

0,4186

GmTUB

0,293

GmUBC2

0,647

GmTUB

1,219

GmTUB

2,638

GmUBC4

0,7903

GmUBC2

0,948

GmTUB

1,246

Best combination

Stability value

Best combination

Stability value

Best combination

Stability value

Best combination

Stability value

Best combination

Stability value

GmELF1B and GmTUA5

0,177

GmACT11 and GmTUA5

0,176

GmACT11 and GmELF1A

0,133

GmCYP2 and GmELF1A

0,057

GmACT11 and GmELF1A

0,084

  1. Stability values are listed from the most stable genes to the least stable.