Figure 3

Efficiency of MNN5 gene amplification from liquid culture of modified strain. A) Percentage of MNN5 amplification from 47 colonies picked on Petri dishes and 47 colonies picked from liquid cultures in micro-plates. B) Growth curve in micro-plate (YPD medium), average of two colonies of YiMMOgène. The OD₆₀₀ was measured in a micro-plate reader. ♦: OD₆₀₀ after 6 hour culture (early exponential phase). ●: OD₆₀₀ after 17 hour culture (late exponential phase). ▄: OD₆₀₀ after 24 hour culture (early stationary phase). C) Percentage amplification of MNN5 from 47 YiMMOgène after different culture times in micro-plate. All amplifications have been carried out with the Platinium Taq.