Table 1 Primers used in this study
From: PCR on yeast colonies: an improved method for glyco-engineered Saccharomyces cerevisiae
Targeted gene | Primer name | Primer sequence | Tm | Product lenght |
|---|---|---|---|---|
MNN5 | CR067-Fw | 5′-ACGACTGGTTCCTAGCACAAC-3′ | 64°C | 900 bp |
CR068-Rv | 5′AAAAGGCGGGAGGGGTGAC-3′ | 62°C | ||
MNN2 | CR064-Fw | 5′-TTCCTCAGACGCCGCCAG-3 | 60°C | 710 bp |
CR064-Rv | 5′GGCCACATGACCAAGCCC-3′ | 60°C |