Table 2 Thermocycling conditions used in this study
From: PCR on yeast colonies: an improved method for glyco-engineered Saccharomyces cerevisiae
Initial denaturation | 95°C | 15 min | 1 cycle |
Denaturation | 95°C | 30 s | 40 cycles |
Annealing | 58°C | 30 s | |
Extension | 72°C | 1 min | |
Final extension | 72°C | 5 min | 1 cycle |