Figure 2

Serum titration on LFA prototype. For testing prototypes, a positive control pool of sera from Ugandan TB patients, all with sputum-confirmed tuberculosis and ELISA-confirmed reactivity against one of more of the test antigens was prepared, aliquoted and frozen. A negative control pool was likewise prepared from Danish donors with no known risk factors for TB and no known exposure, all of whom had been confirmed negative for the test antigen by ELISA. A typical result, showing titration of the positive control pool from 1:1 to 1: 8 (left to right), with diluent-only control in the 5th lane of each test to the right.