Figure 4

No obvious deficit in the social interaction of Grin1^Rgsc174/Grin1⁺mice. In the social interaction test in an open field, there were no significant differences in the number of contacts (A), mean duration of each contact (B), or total duration of contacts (C) between Grin1^Rgsc174/Grin1⁺ and wild-type mice. (D) The total traveled distance was significantly increased in Grin1^Rgsc174/Grin1⁺ mice. In the social interaction test in home cage, there were no significant differences between genotypes in the mean numbers of particles (E) or activity levels (F). Locomotor activity from 5:00 a.m. to 6:00 a.m. was significantly increased in Grin1^Rgsc174/Grin1⁺ mice. (G) In the Crawley’s three-chamber social approach test, there was no significant difference in preference between an empty cage and a cage with a stranger mouse, in either wild-type or in Grin1^Rgsc174/Grin1⁺ mice. (H) No significant differences were detected in total time spent around either the cage with the stranger mouse or the empty cage. (I) The distance traveled was significantly increased in Grin1^Rgsc174/Grin1⁺ mice. In the social novelty preference test, (J) Grin1^Rgsc174/Grin1⁺ mice spent a longer time in the cage with a stranger mouse than with the familiar one, but there was no significant difference between genotypes in the time spent in the cage with the stranger mouse. (K) There was no significant difference in the total time spent around either cage with the familiar mouse or with the stranger one. (L) The total distance traveled by Grin1^Rgsc174/Grin1⁺ mice was significantly greater than that of wild-type mice in the social novelty preference test. The p values indicate the effect of genotype in one-way ANOVA, or in two-way repeated measures ANOVA (E, F) or the paired t-tests (G, J).