Coomassie-stained SDS-PAGE (Phastgel gradient 10-15%) of urinary proteins of two independent experiments performed with Sprague-Dawley male rats fed with high protein diet (n = 3 rats per experiment). The body weights of each animal at bands 1 and 3 first appearance have been highlighted with a square. The mean (± SD) animal weight at bands 1 and 3 first appearance is also indicated per experiment. MW: molecular weight marker. α2u: α2u globulin standard.