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Table 2 q-RT-PCR primer sequences, amplicon size, reaction efficiencies and correlation coefficients in q-RT-PCR assay for the selected references genes and the target genes follicle cell protein 3c and CYP15A1

From: Sequencing and validation of housekeeping genes for quantitative real-time PCR during the gonadotrophic cycle of Diploptera punctata

q-RT-PCR primers Forward Reverse Amplicon size (bp) Efficiency (%) R2
Actin 5'-TCGCACACAGTACCAATCTATGAA-3' 5'-CAAGTCACGACCAGCCAGATC-3' 78 99.3 0.980
EF1-a 5'-TCGTCTTCCTCTGCAGGATGTCT-3' 5'-GGGTGCAAATGTCACAACCATACC-3' 109 99.2 0.994
GAPDH 5'-TCCGTGTGCCTGTTCCTAATG-3' 5'-GCTGCCTTGCCAAGTCTGA-3' 61 100 0.988
Arm 5'-GCTACTGCACCACTCACAGAATTATT-3' 5'-CTGCAGCATACGTTGCAACA-3' 64 94.5 0.980
RpL32 5'-GCGCTTCAAGGGCCAGTAC-3' 5'-TGCTTGGTTTTCTTATTGCTACCA-3' 63 97.4 0.995
SDHa 5'-GCTCTTCTGTGCATGGTGCTAA-3' 5'-GCACGTCCGAACACAACAAG-3' 70 94.6 0.975
AnnIX 5'-CAGAGGTTGGAGATTGCTGA-3' 5'-TGCATCTTCAAATGCTCCTC-3' 96 90.8 0.989
Tub 5'-AAATTACCAACGCTTGCTTTGAA-3' 5'-TGGCGAGGATCGCATTTT-3' 58 95.1 0.993
Fcp 3c 5'-TCTACCTTGCAACGCTTTTG-3' 5'-TCCCTGTCTATTGAGCCACA-3' 111 92.9 0.975
CYP15A1 5'-GTTGGGATCTCGGAGCATGG -3' 5'-CGAACACGTCATGCATCGGT -3' 116 100 0.992