Table 2 q-RT-PCR primer sequences, amplicon size, reaction efficiencies and correlation coefficients in q-RT-PCR assay for the selected references genes and the target genes follicle cell protein 3c and CYP15A1
q-RT-PCR primers | Forward | Reverse | Amplicon size (bp) | Efficiency (%) | R2 |
|---|---|---|---|---|---|
Actin | 5'-TCGCACACAGTACCAATCTATGAA-3' | 5'-CAAGTCACGACCAGCCAGATC-3' | 78 | 99.3 | 0.980 |
EF1-a | 5'-TCGTCTTCCTCTGCAGGATGTCT-3' | 5'-GGGTGCAAATGTCACAACCATACC-3' | 109 | 99.2 | 0.994 |
GAPDH | 5'-TCCGTGTGCCTGTTCCTAATG-3' | 5'-GCTGCCTTGCCAAGTCTGA-3' | 61 | 100 | 0.988 |
Arm | 5'-GCTACTGCACCACTCACAGAATTATT-3' | 5'-CTGCAGCATACGTTGCAACA-3' | 64 | 94.5 | 0.980 |
RpL32 | 5'-GCGCTTCAAGGGCCAGTAC-3' | 5'-TGCTTGGTTTTCTTATTGCTACCA-3' | 63 | 97.4 | 0.995 |
SDHa | 5'-GCTCTTCTGTGCATGGTGCTAA-3' | 5'-GCACGTCCGAACACAACAAG-3' | 70 | 94.6 | 0.975 |
AnnIX | 5'-CAGAGGTTGGAGATTGCTGA-3' | 5'-TGCATCTTCAAATGCTCCTC-3' | 96 | 90.8 | 0.989 |
Tub | 5'-AAATTACCAACGCTTGCTTTGAA-3' | 5'-TGGCGAGGATCGCATTTT-3' | 58 | 95.1 | 0.993 |
Fcp 3c | 5'-TCTACCTTGCAACGCTTTTG-3' | 5'-TCCCTGTCTATTGAGCCACA-3' | 111 | 92.9 | 0.975 |
CYP15A1 | 5'-GTTGGGATCTCGGAGCATGG -3' | 5'-CGAACACGTCATGCATCGGT -3' | 116 | 100 | 0.992 |