- Research article
- Open Access
Hydrogen atoms in protein structures: high-resolution X-ray diffraction structure of the DFPase
© Elias et al.; licensee BioMed Central Ltd. 2013
- Received: 27 March 2013
- Accepted: 27 July 2013
- Published: 2 August 2013
Hydrogen atoms represent about half of the total number of atoms in proteins and are often involved in substrate recognition and catalysis. Unfortunately, X-ray protein crystallography at usual resolution fails to access directly their positioning, mainly because light atoms display weak contributions to diffraction. However, sub-Ångstrom diffraction data, careful modeling and a proper refinement strategy can allow the positioning of a significant part of hydrogen atoms.
A comprehensive study on the X-ray structure of the diisopropyl-fluorophosphatase (DFPase) was performed, and the hydrogen atoms were modeled, including those of solvent molecules. This model was compared to the available neutron structure of DFPase, and differences in the protein and the active site solvation were noticed.
A further examination of the DFPase X-ray structure provides substantial evidence about the presence of an activated water molecule that may constitute an interesting piece of information as regard to the enzymatic hydrolysis mechanism.
- Sub-Ångstrom X-ray crystallography
- Hydrogen atoms
- MoPro software
Organophosphates (OPs) are potent toxic compounds that inhibit acetylcholinesterase, a key enzyme within the central nervous system. These compounds have been extensively used as agricultural pesticides, while their toxic properties have fostered the development of chemical warfare agents such as soman, sarin and VX . Since current methods implemented to remove such compounds prove slow, expensive and generate environmental concerns as well [e.g.; bleach treatment and incineration], enzymes that are capable of detoxifying OPs prove therefore particularly valuable .
Several different protein families have been described as OPs-hydrolases. For instance, microbial phosphotriesterases (PTEs) hydrolyze these compounds with high efficiency, close to the diffusion limit against some OPs, as demonstrated for the PTE from Pseudomonas diminuta. A closely related protein family was recently discovered and labeled Phosphotriesterase–like Lactonase (PLL) . Although its members are natural lactonases which could be involved in the regulation of the quorum sensing [5, 6], these enzymes possess a promiscuous activity against a broad range of phosphotriesters [7, 8]. In Human, the High Density Lipoprotein (HDL) associated human paraoxonase-1 is also capable of hydrolyzing OPs. The structure of a chimeric mammalian recombinant paraoxonase-1 was solved, and displayed a six-bladed ß-propeller with two calcium ions in a central water-filled tunnel . The diisopropyl fluorophosphatase (DFPase) from the squid Loligo vulgaris is able to detoxify a broad range of organophosphorous compounds, including tabun, sarin and soman although its favorite substrate remains the diisopropyl fluorophosphate . The DFPase is a 35 kDa calcium-dependent enzyme that exhibits a six-bladed ß-propeller structure with two calcium ions in a central water-filled tunnel, similar to that of the mammalian recombinant paraoxonase-1 .
The catalytic mechanism of DFPase has been investigated. Blum et al.  elicited the following mechanism based on a H218O isotope labeling experiment: the calcium-coordinated Asp_229 was identified as the nucleophile that attacks the phosphorus center of the substrate via the formation of a phosphoenzyme intermediate. The enzyme would then be regenerated by a solvent water molecule that was not yet identified. Another possible catalytic mechanism for DFPase might involve the activation of a water molecule, as in PTEs , PLLs , human paraoxonase-1 , and the lactonase from Staphylococcus aureus (drug resistance protein 35) , a DFPase structural homologue. Under such conditions, it is the reactive hydroxide ion which acts as a nucleophile, attacking the phosphorus center of the substrates.
The experimental determination of the protonation states of amino acids in the active site pocket would help to discriminate between both mechanisms. The sub-Ångstrom resolution X-ray (0.85Å) structure was previously determined . Congruently, Blum et al., (2009) have performed a neutron diffraction study at 2.2 Å resolution, benefiting thereby from the high diffraction power of the DFPase crystals  and obtained a combined neutron-X-ray (X-N) model. Since both sub-Ångstrom X-ray resolution data and medium resolution neutron data are available for DFPase, the respective structures enables cross-comparison between hydrogen bonds and water networks. In our report hereby, we provide a detailed account regarding the water molecule protonation states within the DFPase active site.
Hydrogen atoms in electron density
Water hydrogen bonds comparison
Active site water
As stated here-above, the orientation of numerous water molecules differs in the X-N and the X-ray structure. Particularly, the active site DOD_1033 is interesting since this molecule coordinates the catalytic calcium cation. Its configuration is unusual, as one of its deuterium atoms points towards the calcium ion with a short contact distance (Hw…Ca2+ distance: 2.0 Å, O-Hw…Ca2+ angle: 111.9°, see Figure five in . As these atoms (H and Ca2+) are both positively charged, the configuration is rather unfavorable.
The analysis of the chemical environment of the water molecule in the X-ray structure shows that HOH_524 is involved in the following interactions (Figure 4): (i) Its hydrogen atom, located with a strong electron density peak described earlier, points towards HOH 511 and forms a canonical hydrogen bond (Ow…Ow distance: 2.82 Å, angle Ow-Hw…Ow = 136°). (ii) Its oxygen atom accepts a hydrogen bond from the NH2 group of the neighboring ASN_175 side chain (HD21…Ow distance: 2.23 Å, angle N-H…Ow: 139°). (iii) The oxygen atom of HOH_524 is coordinated to the catalytic calcium (distance: 2.5 Å) and (iv) further interacts with the carboxylate side chain of Asp_229. The Ow…Oδ2 and Ow…Oδ1 distances are both 2.82 Å, which corresponds to standard hydrogen bond donor-acceptor distance in protein H…O bonds. More precisely, the location of the second hydrogen atom of HOH_524 between Ow and Asp_229 Oδ1 is less favorable, due to the vicinity of the calcium ion. Hence Oδ2 of Asp_229 is the only putative hydrogen bond acceptor atom for HOH_524. In this case, the OW_511…OW_524…Oδ2 angle is 120°, which fits perfectly with the possible hydrogen bond network involving both hydrogen atoms of HOH_524 water molecule. However, despite this ideal geometric configuration, the Fobs-Fcalc density map does not yield significant density peak for a second HOH_524 hydrogen atom interacting with Asp_229.
Discrepancies between X-N and X-ray structures
The hydrogen bond network of water molecules is different in the X-N and X-ray structures, despite the similar localization of water oxygen atoms. In addition, the donor-acceptor (H…O) distances turn out to be shorter in the X-ray structure than in the X-N model. Such discrepancies are interesting and could be due to an intrinsic difference within the information from X-rays or neutrons, to different quality and resolution of the diffraction data sets or to the experimental setups, such as the differences in thermal motion for the water molecules between the two structures (see Additional file 1: Table S1), and the data collection temperature (cryogenic for X-rays, room temperature for neutrons). The last discrepancy is likely to affect the hydration pattern of proteins and the observed differences in solvation may reflect the effect of temperature.
Active site calcium-bound water molecule
As underscored so far, the case of the calcium coordinated water molecule HOH_524 proves indeed noteworthy. First, because of its location close to Asp_229 and its coordination to the catalytic calcium. Secondly, because one of its hydrogen atoms is not clearly visible in the electronic density maps, although the oxygen atom is well confined by numerous interactions. This coordination is very different from the chemically unfavourable coordination of this water molecule in the X-N structure (Ca-O-H angle of 53° and Ca…H distance shorter than the van der Waals contact), that was attributed to the special environment of the protein active site .
Noteworthy, in the X-ray structure, this water molecule sits in a different orientation. As explained above, this observation may be the consequence of different experimental setups like temperature and consequently altered thermal motion parameters. Indeed, significant differences between structures at different temperature have sometimes been noted . It is however interesting to notice that this conformation seems to be chemically more stable than that observed in the X-N structure. Indeed, the hydrogen atom of HOH-524, located with a strong electron density peak, points towards HOH 511 and forms a canonical hydrogen bond. A second interaction is observed with the carboxylate of Asp_229 (2.82 Å), but no significant electron density peak is visible in H omit maps above a 1 sigma level. However, a hydrogen atom could still be present: high atomic thermal motion, multiple conformations or diffuse position such as low-barrier H-bonds [19, 22] can result in weak or even absent electron density for hydrogen atoms. Nevertheless, regarding the other interactions involving HOH-524, and especially the Ow … Asp_229_Oδ2 contact, one could expect both hydrogen atoms to be well defined, and their electron density peaks visible, similarly to the other water hydrogen atoms in the active site. A likely explanation involves a partially deprotonated HOH_524 water molecule. Indeed, Asp_229_Oδ2 may act as a base, accepting a proton from HOH_524, the latter being subsequently activated into a hydroxide ion. Such a mechanism is compatible with the nearby presence of the Ca2+, which acts as a Lewis acid by fostering the HOH_524 proton release (pKa of H2O/ Ca2+ = 12.80 ) and interacting with the corresponding free electron doublet from the activated water molecule. In the framework of this mechanism, the observed electron density results from the occurrence of two protonation states of HOH_524, which may account for the weak electron density peak located between Ow_524 and Asp_229_Oδ2. The elliptical shape of the second hydrogen atom of HOH_524 electron density peak further corroborates such a statement inasmuch as it could reflect a superimposition of the hydrogen atom electron density in both HOH_524 protonation states. The two different states of the water molecule should adopt two slightly different orientations (Figure 4). Thus, findings heretofore suggest that this water molecule is partially deprotonated, with a hydrogen atom in a diffuse position. This observation contrasts with the X-N structure, where the corresponding water molecule is described as a classical H2O molecule .
May the observable partially activated water molecule be involved in the DFPase catalytic mechanism?
The initial mechanism that was proposed for this enzyme consisted in a nucleophilic attack on the phosphorous center of the substrate by an activated hydroxide ion . This mechanism is similar to those proposed for other organophosphorous hydrolases, such as the PTEs and PLLs [6, 25], and the close DFPase structural homologues, the human paraoxonase  as well as the lactonase Drp35 . Later on, however, while resorting to H218O isotope labeling, the calcium-coordinated Asp_229 was thought to act as the nucleophile that attacks the phosphorus center of the substrate, via the formation of a phosphoenzyme intermediate . This new mechanism, along with other reports such as stereospecificity studies , discarded the previous requirement for an activated water molecule. Noteworthy, the X-N structure of DFPase did not reveal the presence of an activated water molecule in the active site . However, if converging elements suggests that Asp_229 is the nucleophile that attacks the phosphorus center, an important issue regarding enzyme regeneration, by a solvent water molecule, remains poorly addressed . Could this observed partially activated water molecule play a role in the enzyme regeneration?
Several observations might speak in favor of this hypothesis. Firstly, a use of this water molecule during catalysis would be consistent with the previous observation that the binding of a substrate mimic to the DFPase yields a completely buried active site . Secondly, the catalytic calcium coordination is plastic in DFPase , but also in structurally-related enzymes such as rHPON  and the gluconolactonase , a key property that may be utilized to reorganize the calcium coordination shell during catalysis. As an illustration, Glu_48 (in gluconolactonase structure, equivalent to Glu_21 for DFPase) adopts the same configuration as elicited in rHPON and DFPase structures: it is bound to the catalytic calcium cation. However, the second monomer of the asymmetric unit, Glu_21 does not interact directly with the catalytic calcium as in DFPase, but only indirectly, performing thereby a short hydrogen bond with a metal-bound water (see Additional file 1: Figure S1). Through a structural comparison between the DFPase, the rHPON and the gluconolactonase, the movement of Glu_21 that occurs in the gluconolactonase seems possible. The important plasticity of these active sites might allow a pre-existing water molecule to be re-located while the substrate binds to the active site. Noteworthy, HOH_524 in DFPase structure sits in a similar location than that of the proposed attacking water molecule in PON1 : it is located in the vicinity of the catalytic calcium and interacts with Asp_269 that co-activates it (Asp_229 in DFPase). Altogether, the presence of a partially activated water molecule in the active site of the sub-Ångstrom resolution structure of DFPase might constitute an interesting piece of information, possibly useful to refine the DFPase mechanism.
The analysis of the high-resolution X-ray structure of DFPase exemplifies that high-resolution X-ray crystallography can be an efficient technique to study hydrogen atoms location, when the data are appropriately refined. Our Sub-Ångstrom X-ray model enabled us to identify numerous water hydrogen atoms and to construct experimentally,--on the sole basis of electron density peaks--, the water hydrogen bonds network in the protein central tunnel. Thorough scrutiny of these H bonds networks in both our model and the available neutron X-N structure reveals different solvation states that may be due to the thermal motion or to experimental setups (e.g. temperature of data collection). They possibly reflect different states existing in solution. Our investigation into the DFPase active site in the X-ray structure suggests that the calcium-coordinated water molecule HOH_524 is partially activated into a hydroxide ion through proton relocation towards the nearby Asp_229 Oδ2 atom.
The starting molecular file in SHELXL format  of the 0.85 Å resolution X-ray structure and the reflection file from an earlier study [11, 17] were kindly provided by Koepke and colleagues. We performed an additional refinement of the DFPase model using MoPro software . Among its distinctive features, MoPro software enables electron density refinement based on a non-spherical atom model , benefiting additionally from the aspherical scattering factor library ELMAM . In the case of DFPase, the resolution of the diffraction data remains inadequate to perform satisfactorily the refinement of the multipolar parameters. Compared to the independent atom model, the number of parameters increases dramatically (from 10 up to 36 par atom) and a sizeable number of observations is required. Hence, the multipolar refinement is often performed locally in low thermal motion protein regions. The isotropic thermal displacement parameters Beq of the atoms in the active site of DFPase are rather low for a protein (Beq of residues in the active site: 5.3 Å2), however not low enough to reach effective multipolar refinement. Test refinements merely yielded unsatisfactory electron density parameters. Therefore, the scaling factor and solvent parameters (using exponential scaling model ) were refined. The quality of electron density maps enabled us to identify density peaks corresponding to some water hydrogen atoms in Fobs-Fcalc residual maps that were placed manually using the Coot program . Proper angle and distance optimization was carried out using the MoPro software so as to comply with both the electron density peaks and the usual stereochemistry of the water molecule. The Hw-Ow-Hw angle for water was constrained at 104.5°. The Ow-Hw bond length in water molecules observed in X-ray structures usually reaches 0.81 Å . However, in order to compare the H-bond network in both the X-ray and X-N structures, the bond length has been elongated to the average value observed by neutron diffraction, namely 0.96 Å . Such a discrepancy occurs as the electron of the hydrogen atom is shared within the covalent bond. Consequently, the X-H distances appear shorter in the X-ray than in the X-N structure. The ordered water molecules are mainly located in the water-filled tunnel of the protein and within the DFPase active site. The structure was deposited in the PDB (id:3O4P).
The model and nuclear structure factors were retrieved from the Protein Data Bank (PDB  id: 3BYC). Structure factor phases for the nuclear density maps computation were obtained after a single refinement cycle (scale factor and solvent parameters) with the Phenix software v.1-4.138 . The applied resolution, weighting schemes and diffraction data cut-offs were the same as those used in the reported DFPase X-N structure .
Analysis of water hydrogen bond geometry
The geometric parameters of the hydrogen bond network formed by the water molecules in the X-ray and X-N structures were analyzed using MoPro software. Only Hw…O hydrogen bonds were investigated, inasmuch as Hw is a water hydrogen atom and O a protein or water oxygen atom. Neighboring atoms which fulfilled required criteria were considered as hydrogen-bonded, i.e. whenever the Hw…O distance was below 2.5 Å and the OwHw …O angle above 90°. In the X-ray structure, the analysis was performed on the water molecules for which the H atoms were placed according to electron density. In the X-N structure, we analyzed the water molecules identified at positions equivalent to those in the X-ray structure. Based on the here-above mentioned criteria for H bonding, thirty and thirty-eight hydrogen bonds were considered for the X-ray and X-N structures, respectively.
This research was supported by grants to E.C. from Délégation Générale pour l’Armement (DGA, REI#09C7002) and from CNRS.
- Raushel FM: Bacterial detoxification of organophosphate nerve agents. Curr Opin Microbiol. 2002, 5: 288-295. 10.1016/S1369-5274(02)00314-4.PubMedView ArticleGoogle Scholar
- Singh BK: Organophosphorus-degrading bacteria: ecology and industrial applications. Nat Rev Microbiol. 2009, 7: 156-164.PubMedView ArticleGoogle Scholar
- Caldwell SR, Newcomb JR, Schlecht KA, Raushel FM: Limits of diffusion in the hydrolysis of substrates by the phosphotriesterase from Pseudomonas diminuta. Biochemistry. 1991, 30: 7438-7444. 10.1021/bi00244a010.PubMedView ArticleGoogle Scholar
- Afriat L, Roodveldt C, Manco G, Tawfik DS: The latent promiscuity of newly identified microbial lactonases is linked to a recently diverged phosphotriesterase. Biochemistry. 2006, 45: 13677-13686. 10.1021/bi061268r.PubMedView ArticleGoogle Scholar
- Del Vecchio P, Elias M, Merone L, Graziano G, Dupuy J, Mandrich L, Carullo P, Fournier B, Rochu D, Rossi M: et al., Structural determinants of the high thermal stability of SsoPox from the hyperthermophilic archaeon Sulfolobus solfataricus. Extremophiles. 2009, 13: 461-470. 10.1007/s00792-009-0231-9.PubMedView ArticleGoogle Scholar
- Elias M, Dupuy J, Merone L, Mandrich L, Porzio E, Moniot S, Rochu D, Lecomte C, Rossi M, Masson P: et al., Structural basis for natural lactonase and promiscuous phosphotriesterase activities. J Mol Biol. 2008, 379: 1017-1028. 10.1016/j.jmb.2008.04.022.PubMedView ArticleGoogle Scholar
- Merone L, Mandrich L, Rossi M, Manco G: A thermostable phosphotriesterase from the archaeon Sulfolobus solfataricus: cloning, overexpression and properties. Extremophiles. 2005, 9: 297-305. 10.1007/s00792-005-0445-4.PubMedView ArticleGoogle Scholar
- Elias M, Tawfik DS: Divergence and convergence in enzyme evolution: parallel evolution of paraoxonases from quorum-quenching lactonases. J Biol Chem. 2012, 287: 11-20. 10.1074/jbc.R111.257329.PubMedPubMed CentralView ArticleGoogle Scholar
- Ben-David M, Elias M, Filippi JJ, Dunach E, Silman I, Sussman JL, Tawfik DS: Catalytic versatility and backups in enzyme active sites: the case of serum paraoxonase 1. J Mol Biol. 2012, 418: 181-196. 10.1016/j.jmb.2012.02.042.PubMedView ArticleGoogle Scholar
- Scharff EI, Koepke J, Fritzsch G, Lucke C, Ruterjans H: Crystal structure of diisopropylfluorophosphatase from Loligo vulgaris. Structure. 2001, 9: 493-502. 10.1016/S0969-2126(01)00610-4.PubMedView ArticleGoogle Scholar
- Koepke J, Scharff EI, Lucke C, Ruterjans H, Fritzsch G: Atomic resolution crystal structure of squid ganglion DFPase. Acta Crystallogr D Biol Crystallogr. 2002, 58: 1757-1759. 10.1107/S0907444902012714.PubMedView ArticleGoogle Scholar
- Blum MM, Lohr F, Richardt A, Ruterjans H, Chen JC: Binding of a designed substrate analogue to diisopropyl fluorophosphatase: implications for the phosphotriesterase mechanism. J Am Chem Soc. 2006, 128: 12750-12757. 10.1021/ja061887n.PubMedView ArticleGoogle Scholar
- Aubert SD, Li Y, Raushel FM: Mechanism for the hydrolysis of organophosphates by the bacterial phosphotriesterase. Biochemistry. 2004, 43: 5707-5715. 10.1021/bi0497805.PubMedView ArticleGoogle Scholar
- Ben-David M, Wieczorek G, Elias M, Silman I, Sussman JL, Tawfik DS: Catalytic metal ion rearrangements underline promiscuity and evolvability of a metalloenzyme. J Mol Biol. 2013, 425: 1028-1038. 10.1016/j.jmb.2013.01.009.PubMedView ArticleGoogle Scholar
- Tanaka Y, Morikawa K, Ohki Y, Yao M, Tsumoto K, Watanabe N, Ohta T, Tanaka I: Structural and mutational analyses of Drp35 from Staphylococcus aureus: a possible mechanism for its lactonase activity. J Biol Chem. 2007, 282: 5770-5780.PubMedView ArticleGoogle Scholar
- Blum MM, Koglin A, Ruterjans H, Schoenborn B, Langan P, Chen JC: Preliminary time-of-flight neutron diffraction study on diisopropyl fluorophosphatase (DFPase) from Loligo vulgaris. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007, 63: 42-45.PubMedPubMed CentralView ArticleGoogle Scholar
- Koepke J, Scharff EI, Lucke C, Ruterjans H, Fritzsch G: Statistical analysis of crystallographic data obtained from squid ganglion DFPase at 0.85 Å resolution. Acta Crystallogr D Biol Crystallogr. 2003, 59: 1744-1754. 10.1107/S0907444903016135.PubMedView ArticleGoogle Scholar
- Howard EI, Sanishvili R, Cachau RE, Mitschler A, Chevrier B, Barth P, Lamour V, Zandt MV, Sibley E, Bon C: et al., Ultrahigh resolution drug design I: Details of interactions in human aldose reductase-inhibitor complex at 0.66 Å. Proteins: Structure, Function, Bioinformatics. 2004, 55: 792-804. 10.1002/prot.20015.View ArticleGoogle Scholar
- Liebschner D, Elias M, Moniot S, Fournier B, Scott K, Jelsch C, Guillot B, Lecomte C, Chabrière E: Elucidation of the Phosphate Binding Mode of DING Proteins Revealed by Subangstrom X-ray Crystallography. J Am Chem Soc. 2009, 131: 7879-7886. 10.1021/ja901900y.PubMedView ArticleGoogle Scholar
- Blum M-M, Mustyakimov M, Rüterjans H, Kehe K, Schoenborn BP, Langan P, Chen JC-H: Rapid determination of hydrogen positions and protonation states of diisopropyl fluorophosphatase by joint neutron and X-ray diffraction refinement. Proc Natl Acad Sci. 2009, 106: 713-718. 10.1073/pnas.0807842106.PubMedPubMed CentralView ArticleGoogle Scholar
- Fraser JS, van den Bedem H, Samelson AJ, Lang PT, Holton JM, Echols N, Alber T: Accessing protein conformational ensembles using room-temperature X-ray crystallography. Proc Natl Acad Sci USA. 2011, 108: 16247-16252. 10.1073/pnas.1111325108.PubMedPubMed CentralView ArticleGoogle Scholar
- Elias M, Wellner A, Goldin-Azulay K, Chabriere E, Vorholt JA, Erb TJ, Tawfik DS: The molecular basis of phosphate discrimination in arsenate-rich environments. Nature. 2012, 491: 134-137. 10.1038/nature11517.PubMedView ArticleGoogle Scholar
- Vrettos JS, Brudvig GW: Water oxidation chemistry of photosystem II. Philos Trans R Soc Lond B Biol Sci. 2002, 357: 1395-1404. 10.1098/rstb.2002.1136. discussion 1404–1395, 1419–1320PubMedPubMed CentralView ArticleGoogle Scholar
- Blum MM, Mustyakimov M, Ruterjans H, Kehe K, Schoenborn BP, Langan P, Chen JC: Rapid determination of hydrogen positions and protonation states of diisopropyl fluorophosphatase by joint neutron and X-ray diffraction refinement. Proc Natl Acad Sci U S A. 2009, 106: 713-718. 10.1073/pnas.0807842106.PubMedPubMed CentralView ArticleGoogle Scholar
- Ghanem E, Raushel FM: Detoxification of organophosphate nerve agents by bacterial phosphotriesterase. Toxicol Appl Pharmacol. 2005, 207: 459-470. 10.1016/j.taap.2005.02.025.PubMedView ArticleGoogle Scholar
- Melzer M, Chen JC, Heidenreich A, Gab J, Koller M, Kehe K, Blum MM: Reversed enantioselectivity of diisopropyl fluorophosphatase against organophosphorus nerve agents by rational design. J Am Chem Soc. 2009, 131: 17226-17232. 10.1021/ja905444g.PubMedView ArticleGoogle Scholar
- Blum M-M, Chen JCH: Structural characterization of the catalytic calcium-binding site in diisopropyl fluorophosphatase (DFPase)–Comparison with related [beta]-propeller enzymes. Chem Biol Interact. 2010, 187: 373-379. 10.1016/j.cbi.2010.02.043.PubMedView ArticleGoogle Scholar
- Chen C-N, Chin K-H, Wang AHJ, Chou S-H: The first crystal structure of gluconolactonase important in the glucose secondary metabolic pathways. J Mol Biol. 2008, 384: 604-614. 10.1016/j.jmb.2008.09.055.PubMedView ArticleGoogle Scholar
- Sheldrick GM: A short history of SHELX. Acta Crystallogr A. 2007, 64: 112-122.PubMedView ArticleGoogle Scholar
- Jelsch C, Guillot B, Lagoutte A, Lecomte C: Advances in protein and small-molecule charge density refinement methods using MoPro. J Appl Cryst. 2005, 38: 38-54. 10.1107/S0021889804025518.View ArticleGoogle Scholar
- Hansen NK, Coppens P: Testing aspherical atom refinements on small-molecule data sets. Acta Crystallogr A. 1978, 34: 909-921. 10.1107/S0567739478001886.View ArticleGoogle Scholar
- Zarychta B, Pichon-Pesme V, Guillot B, Lecomte C, Jelsch C: On the application of an experimental multipolar pseudo-atom library for accurate refinement of small-molecule and protein crystal structures. Acta Crystallogr A. 2007, 63: 108-125. 10.1107/S0108767306053748.PubMedView ArticleGoogle Scholar
- Tronrud DE: TNT refinement package. Macromolecular Crystallography Part B. Volume 277. Edited by: Jr CWC, Sweet RM. 1997, Academic Press, 306-319. Methods in Enzymology http://0-www.sciencedirect.com.brum.beds.ac.uk/science/article/pii/S0076687997770174, http://0-dx.doi.org.brum.beds.ac.uk/10.1016/S0076-6879(97)77017-4View ArticleGoogle Scholar
- Emsley P, Cowtan K: Coot: model-building tools for molecular graphics. Acta Crystallographica Section D. 2004, 60: 2126-2132.View ArticleGoogle Scholar
- Allen FH: A systematic pairwise comparison of geometric parameters obtained by X-ray and neutron diffraction. Acta Cryst B. 1986, 42: 515-522. 10.1107/S0108768186097823.View ArticleGoogle Scholar
- Berman H, Henrick K, Nakamura H: Announcing the worldwide Protein Data Bank. Nat Struct Biol. 2003, 10: 980-10.1038/nsb1203-980.PubMedView ArticleGoogle Scholar
- Adams PD, Afonine PV, Bunkoczi G, Chen VB, Davis IW, Echols N, Headd JJ, Hung L-W, Kapral GJ, Grosse-Kunstleve RW: et al., PHENIX: a comprehensive Python-based system for macromolecular structure solution. Acta Crystallographica Section D. 2010, 66: 213-221. 10.1107/S0108768110007202.View ArticleGoogle Scholar
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