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Table 1 AFB detection and grading in spot-morning-spot sputum specimens using Z-N method

From: Comparative evaluation of a two-reagent cold stain method with Ziehl-Nelseen method for pulmonary tuberculosis diagnosis

ZN; no. (%)/362

Sputum specimen

Grade

3+

2+

1+

Scanty

Negative

Total

1st Spot

8(2.2)

8(2.2)

6(1.7)

2(0.6)

338(93.4)

362(100)

Moring

9(2.5)

7(1.9)

6(1.7)

2(0.6)

338(93.4)

362(100)

2nd Spot

7(1.9)

10(2.8)

5(1.4)

1(0.3)

339(93.6)

362(100)

  1. ZN Ziehl-Neelsen, AFB acid fast bacilli; 3+ = More than 10 AFB per oil immersion field in at least 20 fields; 2+ = 1–9 AFB per oil immersion field in at least 50 fields; 1+ = 10–99 AFB in 100 oil immersion fields; Scanty = 1–9 AFB in 100 oil immersion fields.