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Table 4 Guinea pig reference and target gene information

From: The impact of reference gene selection in quantification of gene expression levels in guinea pig cervical tissues and cells

Gene symbol

Name

Primer sequences 5’ to 3’

Exons

Amplicon size (bp)

Function

Ensembl or GenBank accession no.

Sense and antisense

ACTB

β-Actin

tgcgttacaccctttcttgaca

5

73

Cytoskeletal protein

From ref. [31]

acaaagccatgccaatctcat

ATP5B

ATP synthase, F1 complex β subunit

gatcaatttaaaagatgctacctcgaa

5 and 6

68

ATP synthesis

DQ403103

caccaggcggttcattcatt

ATP6

Adenosine triphosphatase 6

cccactatgagcagcaactgtaa

1

67

ATP metabolism

NC_000884

gaagtgggctagggatgcttt

B2M

β2-Microglobulin

tggtgcatgctgcctttaca

2

64

Cell surface molecule component

NM_001172856

gtgatgtgtgaaactctgcaagaa

CFL1

Cofilin 1

ttccaaggatgccatcaaaaa

3 and 4

65

Actin modification

ENSCPOT00000008138

cgtagcaatttgcctgtaattcg

CLTC

Clathrin

caattcgttttcaggagcatctc

1 and 2

64

Coated vesicle formation protein

ENSCPOT00000005567

aagccaatgtttgctgggtta

CTBP1

C-terminal binding protein 1

tctcatcaacgacttcactgtcaa

4 and 5

61

Transcriptional repressor phosphoprotein

ENSCPOT00000019529

ggccgtgttcaccaggaa

GAPDH

Glyceraldehyde-3-phosphate dehydrogenase

tcagagggctccctcaaag

2

70

Glycolytic enzyme

From ref. [30]

cgctgttgaagtcacaggac

HMBS

Hydroxymethyl-bilane synthase

cctgggttggcagaacaga

9 and 10

66

Heme biosynthesis

ENSCPOT00000006534

tggcccacagcatacatacag

PPIB

Cyclophilin B

gggcctaaagtcaccgtcaa

1 and 2

63

Protein folding catalyst

ENSCPOT00000000258

ccggcccacatcttcatct

RPLP0

Ribosomal protein, large, P0

atgctgctggccaataaggt

3 and 4

64

Protein synthesis

ENSCPOT00000019555

tgacttcacatggtgcaatgg

SDHA

Succinate dehydrogenase, subunit A

gatgccatccattacatgacaga

4 and 5

67

Citric acid cycle enzyme

DQ402978

gcatgccataattttctagctcaa

TBP

TATA-binding protein

acttgacctaaagacaattgcacttc

5 and 6

65

Transcription factor

ENSCPOT00000001200

cagcaaaccgcttgggatta

TFRC

Transferrin receptor

gaccttccagtcttcggtcatg

8 and 9

68

Iron transport

S81327

aaagaagggaacccaggtgtataa

TPT1

Tumor protein, translationally controlled 1

ccttgctaatttcaaaaactatcagttc

4 and 5

67

 

EU330893

agcaaccatgccatctggat

PTGS2

Cyclooxygenase-2

ctgcgcaatgcaatcatga

3 and 4

67

Prostaglandin synthesis

Y07896

agttggtggactgtcgatcaga