Figure 5
Effect of culture temperatures on freezing tolerance induced by exogenous ABA (A) and growth (B) of rice suspension cells. Cell cultures (1 g cell inoculum in 50 mL medium) were incubated at 25, 28 or 30°C for 7 days with or without 75 μM ABA. Freeze survival was determined by regrowth and cell growth on fresh weight basis was measured as detailed in the Methods. Values indicated by different letters (a-c) significantly differed at 5% level using Tukey-Kramer multiple comparison analyses.