Figure 1

ER stress in DENV 2 infected HepG2 cells. (a) HepG2 cells were either infected with DENV 2 at m.o.i. 1 or 10 or mock infected and examined for the percentage of infected cells at 24 hours post infection by flow cytometry. (b) and (c) HepG2 cells were either infected at m.o.i. 10 or mock infected or treated with tunicamycin and after the times indicated examined for the expression of (b) XBP-1 and actin by RT-PCR or (c) GRP78 by western blotting.