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Table 2 Reference genes ranked from the most to the least stable considering CYP-infected Macrosteles quadripunctulatus samples

From: Selection of reference genes from two leafhopper species challenged by phytoplasma infection, for gene expression studies by RT-qPCR

Ranking order

BestKeeper (N = 9)

geNorm (N = 12)

Normfinder (N = 12)

 

Gene name

Std dev [± CP]

Gene name

M value

Gene name

Stability value

1

GAPDH

0.77

ATP Syn β *

1.868

Tropomyosin

0.708

2

Tropomyosin

1.22

Tropomyosin

1.894

ATP Syn β

1.071

3

18S

1.24

GAPDH *

1.919

GAPDH

1.151

4

ATP Syn β

1.43

18S

2.516

18S

1.566

5

Actin

2.13

Actin

2.873

Actin

2.381

  1. *Best combination of two genes according with geNorm (average expression stability M = 1.087).
  2. Best combination of two genes according with Normfinder (stability value = 0.895).
  3. In bold geNorm M values > 1.5.