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Table 5 Reference genes ranked from the most to the least stable considering CYP-infected Euscelidius variegatus samples

From: Selection of reference genes from two leafhopper species challenged by phytoplasma infection, for gene expression studies by RT-qPCR

Ranking order

BestKeeper (N = 13)

geNorm (N = 13)

Normfinder (N = 13)

 

Gene name

Std dev [± CP]

Gene name

M value

Gene name

Stability value

1

GAPDH

0.68

Actin *

0.963

Tropomyosin

0.149

2

18S

0.77

Tropomyosin *

1.016

Actin

0.167

3

ATP Syn β

0.96

ATP Syn β

1.091

GAPDH

0.170

4

Actin

1.20

GAPDH

1.105

ATP Syn β

0.171

5

Tropomyosin

1.33

18S

1.345

18S

0.238

  1. *Best combination of two genes according with geNorm (average expression stability M = 0.517).
  2. Best combination of two genes according with Normfinder (stability value = 0.094).
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BMC Research Notes

ISSN: 1756-0500

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