Figure 1

Analysis flowchart. Schematic overview of the analysis pipeline, and numbers of genes/proteins detected by each technology (top). With each technology, raw data are mapped to a suitable reference. In case of LC-MS/MS, the raw data is compared to known Uniprot/TrEMBL proteins. With RNA-seq, the reads are aligned to the Zv9 genome. Microarray probes were annotated with a known Ensembl gene or transcript. To make comparisons across technologies possible, the protein and array annotations were translated to Ensembl genes. The Venn diagram (bottom) shows the overlaps amongst the genes detected by the different technologies. Green: Illumina RNA-seq, Blue, Agilent microarray and Red, proteomic data from LC-MS/MS.