Clinical utility of RD1, RD9 and hsp65 based PCR assay for the identification of BCG in vaccinated children

Teo, Jeanette WP; Cheng, Janet WS; Jureen, Roland; Lin, Raymond TP

doi:10.1186/1756-0500-6-434

BMC Research Notes

Table 1 Characteristics of specimens sent for BCG identification, clinical MTB isolates and reference strains

From: Clinical utility of RD1, RD9 and hsp65 based PCR assay for the identification of BCG in vaccinated children

Samples	Disease description and management	Specimen site	PCR	result	PCR	oxyR *	SD TB Ag MPT64 Rapid test	AccuProbe Complex test
			RD1	RD9	hsp65
For BCG identification ( n =16)
Case/Sex/Age (months)
1/M/3	BCG adentitis. Excision of inguinal lymph node	Inguinal lymph node	―	―	+	M. bovis	NEG	ND
2/M/2	Incision and drainage of abscess	Axillary abscess	―	―	+	M. bovis	NEG	POS
3/M/2	No information available	Axillary abscess	―	―	+	M. bovis	NEG	POS
4/F/4	Incision and drainage of gluteal abscess	Injection site abscess	―	―	+	M. bovis	NEG	POS
5/M/3	BCG adentitis. Excision of inguinal lymph node	Inguinal lymph node	―	―	+	M. bovis	NEG	POS
6/F/3	No information available	Inguinal lymph node	―	―	+	M. bovis	NEG	POS
7/M/3	No information available	Inguinal lymph node	―	―	+	M. bovis	NEG	POS
8/F/3	No information available	Inguinal lymph node	―	―	+	M. bovis	NEG	POS
9/M/4	BCG adenitis. Excision of inguinal lymph node	Injection site abscess	―	―	+	M. bovis	NEG	POS
10/M/22	No information available	Lymph node	―	―	+	M. bovis	NEG	POS
11/M/2	No information available	Lymph node	―	―	+	M. bovis	NEG	POS
12/F/3	Left caseating inguinal lymph node.	Lymph node	―	―	+	M. bovis	NEG	POS
13/M/3	No information available	Inguinal lymph node	―	―	+	M. bovis	NEG	POS
14/M/4	Left axillary lymph node abscess. Incision and drainage	Axillary abscess	―	―	+	M. bovis	NEG	POS
15/M/3	No information available	Lymph node abscess	―	―	+	M. bovis	NEG	POS
16/M/3	No information available	Inguinal lymph node aspirate	―	―	+	M. bovis	NEG	POS
For specificity and sensitivity testing
Clinical MTB isolates (n= 32)		Respiratory and non-respiratory	+	+	+	ND	POS	POS
M. tuberculosis complex control strains (n=3)
M. bovis BCG Pasteur ATCC 35734		NA	―	―	+	M. bovis	NEG	POS
M. tuberculosis H37Ra ATCC 25177		NA	+	+	+	MTB	POS	POS
M. tuberculosis H37Rv ATCC 27294		NA	+	+	+	MTB	POS	POS
Non M. tuberculosis strains (n=13)
M. intracellulare ATCC 13950		NA	―	―	―	ND	ND-	ND
M. gordonae ATCC 35756		NA	―	―	―	ND	ND	ND
M. kansasii ATCC12478		NA	―	―	―	ND	ND	ND
M. septicum ATCC 700731		NA	―	―	―	ND	ND	ND
M. senegalense ATCC 35755		NA	―	―	―	ND	ND	ND
M. perigrinum ATCC 23001		NA	―	―	―	ND	ND	ND
M. xenopi ATCC 19250		NA	―	―	―	ND	ND	ND
M. abscessus ATCC 19977		NA	―	―	―	ND	ND	ND
M. chelonae ATCC 19539		NA	―	―	―	ND	ND	ND
M. fortuitum ATCC 6841		NA	―	―	―	ND	ND	ND
M. haemophilum ATCC 29548		NA	―	―	―	ND	ND	ND
Rhodococcus equi		NA	―	―	―	ND	ND	ND
Norcardia farcinica		NA	―	―	―	ND	ND	ND

ND: Not done.
NA: Not applicable.
— : No PCR amplification observed.
+ : Positive PCR amplification.
oxyR: A single nucleotide polymorphism, G➔A at position 285, revealed by oxyR sequencing differentiates M. bovis and TBC.

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ISSN: 1756-0500

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