Skip to main content

Table 1 Frequency of regeneration of plants from tobacco explants putatively transformed with pMarkfree5.0 and cultured on medium containing kanamycin (100 mg/L)

From: A new double right border binary vector for producing marker-free transgenic plants

Experiment

Total number of explants

Number of regenerating explants

1

37

22

2

36

23

3

36

16

4

31

17

Total

140

78

  1. Regeneration frequency was 55.7%, expressed as total number of regenerating explants per 100 explants infected with LBA4404 (pMarkfree5.0).