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Figure 1 | BMC Research Notes

Figure 1

From: Validation of miRNA-mRNA interactions by electrophoretic mobility shift assays

Figure 1

Binding between miR-224 and the 3′-UTR of the gene SLC4A4 . (A) Lane 1 corresponds to the probe alone (miR-224*), lanes 2 and 3 correspond to the incubation of the probe with increasing concentrations of SLC4A4 3′-UTR (3 and 10 μM), lanes 4 and 5 correspond to the incubation of the probe with increasing concentrations (3 and 10 μM) of mutant SLC4A4 3′-UTR, lane 7 corresponds to the incubation of the probe with tRNA and lanes 8 and 9 correspond to the probe alone, for duplicate, on a denaturing gel. In the lower panel, the binding is measured by the increase in the intensity of the band corresponding to miR-224*:SLC4A4 3′-UTR and it is expressed as the percentage of intensity with respect to the total radioactivity in each lane after quantification with ImageQuant software. (B) Structure prediction of miR-224 conformations.

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