Figure 3
From: Metastatic melanoma in an esophagus demonstrating Barrett esophagus with high grade dysplasia
Pyrosequencing results demonstrate the presence of a 1799T>A mutation in codon 600 of the BRAF gene. The pyrogram shows a CAC>CTC change involving the second nucleotide position of codon 600, highlighted in yellow, in the reverse strand. Allele quantification reveals 68% mutant allele in the tumor sample. This nucleotide change results in a valine to glutamic acid substitution at amino acid position 600.