(A) Growth of Fusarium oxysporum f.sp. conglutinans (left) and Rhizoctonia solani (right) 4 days after inoculation in static liquid culture (L), on solid media without membrane overlay (S) or solid media with nylon membrane overlay (M). Growth of F. oxysporum in static liquid culture was poor and even after prolonged incubation a coherent mycelial mat was never formed. In contrast, growth of R. solani in static liquid culture was such that a coherent mycelia mat was obtained, which could easily be removed using a pair of tweezers. Growth of the replicate cultures was comparable and only one example is shown here. (B) Integrity (upper panels) and yield (lower panels) of total RNA samples isolated from F. oxysporum (left) and R. solani (right). 5 μg of total RNA were separated on a 1.3% formaldehyde agarose gel. M – 0.5-10 kb RNA Ladder (Invitrogen, Mulgrave, VIC, Australia). 1 – static liquid culture, 2 – shaking liquid culture, 3 – solid media, 4 – solid media with membrane overlay, 5 – solid media with membrane overlay and two chloroform extractions.