Figure 4

Surface-attached probe response to 50mer targets with complementary stretch from 6 to 21 nt. Here we show a comparison between probe response to targets with centrally located matches (central shown in blue) with targets with matches located in 3’ end or 5’ end (red). The dotted line represents average background across all the targets used and the solid line represents probe response to its 50mer perfect match. Error bars indicate the standard deviation among replicated arrays.