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Figure 1 | BMC Research Notes

Figure 1

From: Optimization of a high-throughput whole blood expression profiling methodology and its application to assess the pharmacodynamics of interferon (IFN) beta-1a or polyethylene glycol-conjugated IFN beta-1a in healthy clinical trial subjects

Figure 1

Comparative analysis of the relative effects of experimental variables on gene expression profiles. A) An initial filter to remove all qualifiers that changed less than 1.5 fold from the median value in 20% or less of the samples was applied to the dataset. The remaining 13,898 qualifiers were then subjected to hierarchical clustering by correlation with complete linkage. The resultant clustering reflects the experimental conditions that were used in this study. B) In initial stringent filter was applied to remove all qualifiers that changed less than 2 fold between human brain reference RNA and universal human reference RNA and a LODS score of >0. The remaining 7128 qualifiers were then subjected to hierarchical clustering using a Euclidean distance measure with single linkage. The experimental conditions are reflected in the clustering dendrogram.

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