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Figure 5 | BMC Research Notes

Figure 5

From: Ontogeny of adrenal-like glucocorticoid synthesis pathway and of 20α-hydroxysteroid dehydrogenase in the mouse lung

Figure 5

21-hydroxylase protein in the mouse developing lung. A Western blot analysis of the 21-hydroxylase protein is presented for whole lung protein extracts (50 μg) (panel A) and microsomal fractions (50 μg of microsomal proteins) (panel B) for the indicated developmental times. For the study of total protein extracts, levels of Hsp90β are shown as a loading control. Total protein extracts were prepared with the lungs from one individual for each time point, whereas microsomal fractions were prepared using a pool of lungs from all the fetuses/neonates of one litter for each time point. Microsomes from adult adrenals were used as control. The proportion of adrenal microsomal proteins loaded on the gel compared to the amount loaded for the lungs is indicated (1/1000 = 50 ng microsomal proteins, 1/500 = 100 ng, and 1/50 = 1 μg). Each pannel corresponds to a different gel.

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