Figure 3From: Identification of suitable reference genes for hepatic microRNA quantitationGeNorm plus was used to determine (A) the most stable candidate/s as ranked based on M value, and (B) the optimal number of endogenous controls. A normalization factor NF is calculated for at least 2 candidates starting from the most stable pair and the next most stable candidate/s are sequentially added to calculate additional NFs until the average pairwise variation between two sequential normalization factors (V = NFn/NFn+1, where n = number of controls used) falls below a set threshold (0.15).Back to article page