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Figure 3 | BMC Research Notes

Figure 3

From: Identification of suitable reference genes for hepatic microRNA quantitation

Figure 3

GeNorm plus was used to determine (A) the most stable candidate/s as ranked based on M value, and (B) the optimal number of endogenous controls. A normalization factor NF is calculated for at least 2 candidates starting from the most stable pair and the next most stable candidate/s are sequentially added to calculate additional NFs until the average pairwise variation between two sequential normalization factors (V = NFn/NFn+1, where n = number of controls used) falls below a set threshold (0.15).

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