Table 1 Canonical mature miRNAs selected for qPCR validation
From: Analysis options for high-throughput sequencing in miRNA expression profiling
miRNA | HTS (A) | qPCR target sequence | |
|---|---|---|---|
Abundance class | RPM | ||
hsa-miR-27b-5p | Low1 (L1) | 33 | agagcuuagcugauuggugaac |
hsa-miR-708-3p | Low2 (L2) | 26 | caacuagacugugagcuucuag |
hsa-miR-30e-5p | Medium1 (M1) | 3639 | uguaaacauccuugacuggaag |
hsa-miR-146a-5p | Medium2 (M2) | 1002 | ugagaacugaauuccauggguu |
hsa-miR-181a-5p | High1 (H1) | 28011 | aacauucaacgcugucggugagu |
hsa-miR-143-3p | High2 (H2) | 36537 | ugagaugaagcacuguagcuc |