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Figure 1 | BMC Research Notes

Figure 1

From: Identification and survival studies of Mycobacterium tuberculosis within Laboratory-Fermented bovine milk

Figure 1

Detection of milk isolate as M. tuberculosis by Genus- and species-specific PCR. (a) Multiplex PCR with primers Mycgen-F, Mycgen-R and Mycav-R. Lane 1: 1 Kb Plus DNA ladder, lane 2: milk isolate, lanes 3-5: positive controls with M. tuberculosis, M. bovis and M. avium DNA respectively, lane 6: negative control. (b) Multiplex PCR with RD4 (lanes 2-5) and RD10 (lanes 6-9) primers. Lane 1: 1 Kb Plus DNA ladder, lane 2: milk isolate, lanes 3 and 4: positive controls with M. tuberculosis and M. bovis DNA respectively, lane 5: negative control. Lane 6: milk isolate, lanes 7 and 8: positive controls with M. tuberculosis and M. bovis DNA respectively, lane 9: negative control. Molecular weight ladder in b same as in a.

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