Table 1 Purification of β-sitosterol (1) from crude soybean oil extract
From: Rapid purification of gram quantities of β-sitosterol from a commercial phytosterol mixture
Purification step | Fraction | Stigmasterol | β-Sitosterol | Campesterol |
|---|---|---|---|---|
(1) | (2) | (3) | ||
[% of total phytosterols] | ||||
Crude soybean oil extract (S5753, Sigma-Aldrich) | ||||
n.a. | 9.3 | 60.7 | 27.8 | |
Fractional crystallization | ||||
Acetone | S | 8.5 | 51.2 | 40.3 |
L | 12.3 | 57.9 | 29.8 | |
Hexane/toluene/ | S | 12.3 | 57.7 | 30.1 |
ethanol (4:2:1) | L | 4.5 | 66.0 | 29.5 |
Diethylether | S | 12.4 | 61.5 | 26.2 |
L | 3.0 | 58.1 | 39.0 | |
Silica column chromatography | ||||
S | 7.1 | 78.2 | 14.7 | |
L | 1.5 | 82.0 | 16.5 | |
Na-Y zeolite chromatography | ||||
S | 4.1 | 92.2 | 3.7 | |
L | 0.1 | 94.2 | 5.7 | |