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Classical dynamin DNM1 and DNM3 genes attain maximum expression in the normal human central nervous system
BMC Research Notes volume 7, Article number: 188 (2014)
Dynamin is a super-family of large GTPase proteins that polymerise during their biological activity. Dynamin polymers form around lipid tubes and contribute to the membrane fission and scission of nascent vesicles from parent membranes. Here we used the NCBI Gene Expression Omnibus (GEO) database and the BioGPS gene expression portal to study differential dynamin gene expression in normal human organs or tissues. From the GDS1096 and GDS596 dataset, we downloaded the relative expression levels of dynamin-related genes (presented as percentages), with respect to all of the other genes on the array (platform Affymetrix GPL96), which includes the best characterised human genes. The expression profiles of dynamin in the central nervous system (CNS) are clearly distinct from the expression profiles in the other organs or tissues studied. We found that the classical dynamin DNM1 and DNM3 genes reach their maximum expression levels (100% of maximal expression) in all normal human CNS tissues studied. This is in contrast to the expression profile in the other normal human organs or tissues studied, in which both dynamin DNM1 and DNM3 genes showed approximately 50% maximal expression. This data mining analysis supports the concept that there is a relationship between the synapse and the molecular function of dynamin, suggesting a new field of work in the study of neurodegenerative diseases.
We have focused our attention on the expression profiling of dynamin genes in several normal human organs or tissues, using the NCBI Gene Expression Omnibus (GEO) database. We have chosen to study dynamin genes because dynamin is a super-family of GTP-binding mechanochemical proteins that are involved in fundamental processes, such as the scission of nascent vesicles from parent membranes and mitochondrial fusion and/or fission. In addition, we believe that the dynamin super-family is a biological system suitable for studying differential gene expression in organs or tissues.
Specifically, we considered the group of genes coding the following protein families within the dynamin super-family: (i) the classical dynamins and dynamin-like proteins that tubulate and sever membranes and are involved in clathrin-mediated endocytosis and other vesicular trafficking processes (DNM1, DNM1L, DNM2, DNM3); (ii) the guanylate-binding proteins, which are induced by type II interferons and anti-inflammatory cytokines (GBP1 and GBP2); (iii) the proteins on the outside of the outer mitochondrial membrane that participate in mitochondrial fusion and contribute to the maintenance and function of the mitochondrial network (MFN1 and MFN2); (iv) proteins in the mitochondrial inter membrane space that are involved in mitochondrial fusion, and in which mutations in the gene are associated with optic atrophy type 1 (OPA1); and (v) Mx proteins, which are involved in resistance against viral infections (MX1 and MX2). Taken together, these proteins constitute a large set of GTPase proteins that polymerise within each group or family according to the dynamin molecular function. These dynamin polymers (such as rings and helices) are formed around lipid tubes and contribute to membrane fission.
To address this issue, we relied on the independent studies by Su A.I. et al. (GDS596) and Ge X. et al. (GDS1096). We downloaded the relative expression levels of dynamin-related genes (presented as percentages) with respect to all other genes on the array from these NCBI-GEO database records. It is important to note that both NCBI-GEO data sets were created based on the same array platform (Affymetrix GPL96), which include all the best characterised human genes. Figure 1 shows the profiles of dynamin gene expression levels (expressed as percentage and presented as beam shaped lines) in samples from normal human organs or tissues that are common to the NCBI-GEO records of both studies. From a strict visual analysis of these beam lines, the data can be separated into two consistent populations or sub-beam lines of expression profiles, one of which groups together the profiles corresponding to components of the central nervous system (CNS) (Figure 2). This data mining analysis allowed us characterise dymanin gene expression trends in the CNS. We found clear evidence that the classical dynamin DNM1 and DNM3 genes reach their maximum expression level (100% measurement score) in all of the normal human CNS tissues studied. Moreover, this results is in contrast to the expressionprofiles observed in the other normal human organs and tissues studied, in which both the dynamin DNM1 and DNM3 genes showed approximately 50%. A detailed breakdown of the data is presented in Table 1. To improve the rigor of our analysis, we used the BioGPS gene expression portal[7, 8] to explore the expression profiles of human dynamin-retaled genes. We found that the classical dynamin DNM1 and DNM3 genes are also maximally expressed in normal human CNS components and at significantly lower level in others organs or tissues[9, 10].
The CNS consists of the brain and spinal cord, where synapses are the dynamic structures through which all nervous system signals traverse. Dynamin polymerisation in membrane fission is thought to play a significant role in the synapse (Gene Ontology GO:0003373) which is suggestive of the concept of a relationship between synapses and dynamins. Several animal model studies have shown that dynamin genes are highly expressed in neurons[12–15]. In conclusion, based on their high levels of expression in human CNS, DNM1 and DNM3 can be considered as CNS-specific dynamin genes, at least in the following human CNS components: amygdala, foetal brain, whole brain, caudate nucleus, cerebellum, cerebellum peduncles, cingulate cortex, globus pallidus, hippocampus, hypothalamus, medulla oblongata, occipital lobe, parietal lobe, pineal day, pineal night, pons, prefrontal cortex, spinal cord, subthalamic nucleus, temporal lobe, and thalamus. We suggest that the role of dynamins in the CNS could be a potentially interesting area for further biochemical research in neurodegenerative diseases.
Praefcke GJK, McMahon HT: The dynamin superfamily: universal membrane tubulation and fission molecules?. Nature Rev Mol Cell Biol. 2004, 5: 133-147. 10.1038/nrm1313.
Dynamin Hope Page.http://www.endocytosis.org/Dynamin/,
Hinshaw JE, Schmid SL: Dynamin self-assembles into rings suggesting a mechanism for coated vesicle budding. Nature. 1995, 374: 190-192. 10.1038/374190a0.
Sever S, Chang J, Gu C: Dynamin rings: not just for fission. Traffic. 2013, 14: 1194-1199. 10.1111/tra.12116. doi:10.1111/tra.12116
Su AI, Wiltshire T, Batalov S, Lapp H, Ching KA, Block D, Zhang J, Soden R, Hayakawa M, Kreiman G, Cooke MP, Walker JR, Hogenesch JB: A gene atlas of the mouse and human protein-encoding transcriptomes. Proc Natl Acad Sci U S A. 2004, 101: 6062-6067. 10.1073/pnas.0400782101.
Ge X, Yamamoto S, Tsutsumi S, Midorikawa Y, Ihara S, Wang SM, Aburatani H: Interpreting expression profiles of cancers by genome-wide survey of breadth of expression in normal tissues. Genomics. 2005, 86: 127-141. 10.1016/j.ygeno.2005.04.008.
Wu C, Orozco C, Boyer J, Leglise M, Goodale J, Batalov S, Hodge CL, Haase J, Janes J, Huss JW, Su AI: BioGPS: an extensible and customizable portal for querying and organizing gene annotation resources. Genome Biol. 2009, 10: R130-10.1186/gb-2009-10-11-r130.
Wu C, MacLeod I, Su AI: BioGPS and MyGene.info: organizing online, gene-entric information. Nucl Acids Res. 2013, 41 (D1): D561-D565. 10.1093/nar/gks1114.
DNM1 default BioGPS gene report.http://biogps.org/#goto=genereport&id=1759,
DNM3 default BioGPS gene report.http://biogps.org/#goto=genereport&id=26052,
Watanabe S, Rost BR, Camacho-Pérez M, Davis MW, Söhl-Kielczynski B, Rosenmund C, Jorgensen EM: Ultrafast endocytosis at mouse hippocampal synapses. Nature. 2013, 504: 242-247. 10.1038/nature12809. doi:10.1038/nature12809
Cook T, Mesa K, Urrutia RJ: Three dynamin-encoding genes are differentially expressed in developing rat brain. J Neurochem. 1996, 67: 927-931.
Gray NW, Fourgeaud L, Huang B, Chen J, Cao H, Oswald BJ, Hemar A, McNiven MA: Dynamin 3 is a component of the postsynapse, where it interacts with mGluR5 and Homer. Curr Biol. 2003, 13: 510-515. 10.1016/S0960-9822(03)00136-2.
Ferguson SM, Brasnjo G, Hayashi M, Wolfel M, Collesi C, Giovedi S, Raimondi A, Gong LW, Ariel P, Paradise S, O’Toole E, Flavell R, Cremona O, Miesenböck G, Ryan TA, De Camilli P: A selective activity-dependent requirement for dynamin 1 in synaptic vesicle endocytosis. Science. 2007, 316: 570-574. 10.1126/science.1140621.
Raimondi A, Ferguson SM, Lou X, Armbruster M, Paradise S, Giovedi S, Messa M, Kono N, Takasaki J, Cappello V, O’Toole E, Ryan TA, De Camilli P: Overlapping role of dynamin isoforms in synaptic vesicle endocytosis. Neuron. 2011, 70: 1100-1114. 10.1016/j.neuron.2011.04.031.
This study was supported by a grant from the “Ministerio de Economia y Competitivdad” of the Spanish Government (AGL 2008-00387/ALI).
The authors declare that they have no competing interests.
AR carried out the downloading of gene expression data from the NCBI-GEO database, helped design the study, and contributed to writing the manuscript. LA wrote the manuscript and helped design the study. Both authors read and approved the final manuscript.
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Romeu, A., Arola, L. Classical dynamin DNM1 and DNM3 genes attain maximum expression in the normal human central nervous system. BMC Res Notes 7, 188 (2014). https://0-doi-org.brum.beds.ac.uk/10.1186/1756-0500-7-188
- Central nervous system
- Gene expression
- Data mining