Figure 2From: Butyrate induced changes in Wnt-signaling specific gene expression in colorectal cancer cellsOverexpression of DN-Tcf4 in induced HCT-116 cells. A stably transfected clone of HCT-116 cells was treated with 4Ā Ī¼g/ml doxycycline (Doxy) and/or 5Ā mM NaB for 17.5Ā hr or left untreated. Protein was isolated, and DN-Tcf4 detected with anti-FLAG antibody in western blot analysis. Actin was analyzed as a control. Duplicate samples from a representative western blot shown.Back to article page