Development and characterization of 16 polymorphic microsatellite markers from Taiwan cow-tail fir, Keteleeria davidiana var. formosana (Pinaceae) and cross-species amplification in other Keteleeria taxa

Ho, Chin-Shang; Shih, Huei-Chuan; Liu, Ho-Yih; Chiu, Shau-Ting; Chen, Mei-Hui; Ju, Li-Ping; Ko, Ya-Zhu; Shih, Yu-Shen; Chen, Chaur-Tzuhn; Hsu, Tsai-Wen; Chiang, Yu-Chung

doi:10.1186/1756-0500-7-255

Table 2 Summary of general information for the 16 polymorphic microsatellite loci isolated from Keteleeria davidiana var. formosana and their transferability to six Keteleeria taxa

From: Development and characterization of 16 polymorphic microsatellite markers from Taiwan cow-tail fir, Keteleeria davidiana var. formosana (Pinaceae) and cross-species amplification in other Keteleeria taxa

Locus	Repeat motif	Primer sequence(5′-3′)	Allele size (bps)	Ta(°C)							Genbank accession no.	Cross amplification
			Allele size (bps)	TW	cy	pu	ca	cp	fo	ev	Genbank accession no.	Cross amplification
TW-5	(TG)₁₅	F: TTGTCTAGGTATTTGTGCCC	116	54	54	54	54	54	54	54	HG518488	7/7
		R: AAACGCAGTATAAGACAGGC
TW-49	(CTCC)₉	F: TCACTCCCTATATCCCTAGC	99	50	50	50	50	50	50	50	HG518489	7/7
		R: GGAGAGAGAGATTGTGTTTG
TW-69	(CT)₂₇(TATTTGA)₃	F: CCTATATCCTCCCCCACCT	260	54	54	54	54	54	54	54	HG518490	7/7
		R: AGGTATAGAGGGGGATAGTG
TW-107	(CA)₄(AG)₁₄	F: AACCAAGGATGAAACCCTAG	153	54	54	54	54	54	54	54	HG518491	7/7
		R: AAGTGAAAGACATAGTGAGG
TW-172	(GA)₃₀	F: AGACAAAGGAGAATGGTGGG	165	50	50	50	50	50	50	50	HG518492	7/7
		R: CCATGCCATGGGTAAGATAG
TW-195	(CT)₁₈	F: ATCTTCATCACTCTCCCACC	170	54	54	54	54	54	54	54	HG518493	7/7
		R: AAGGGGAAAGTGAGAGAGGG
KE-6	(AC)₁₅	F: ACGCGAGGTTTCGGGTGGCA	192	54	54	54	54	54	54	54	HG518494	7/7
		R: CTGGGTACTGGTGCCCTGT
KE-54	(AC)₁₅	F: ACCTTGCAAACTCACCAGCT	271	52	52	52	52	52	52	52	HG518495	7/7
		R: CTATGGCTAGGTTTCCTGGGT
TCC-6	(AAC)₉	F: TGTATGGTGTGTCAGATGC	342	56	56	56	56	56	56	56	HG518496	7/7
		R: CCTCCTTGTGGAGTTGGCA
TCC-31	(TTG)₅(TTC)₆	F: CTTCAAAGCTGCTCGACTC	285	55	55	55	55	55	55	55	HG518497	7/7
		R: GTTTGCTTGAGGAACAGGT
TCC-55	(GAA)₆(CAA)₁₂	F: CTCTTGAAGAACAGGTTGAG	288	54	54	54	54	54	54	54	HG518498	7/7
		R: CTTCAAAGCTGCTCGGCTCT
TCC-139	(GAA)₁₃	F: CCTACAAGGTTGACCCCGT	220	54	54	54	54	54	54	54	HG518499	7/7
		R: TGCTCGCCAATGCATGACAAG
TCC-245	(GAA)₁₃	F: ACGTTTGAGGAGCAGGTTG	255	50	50	50	50	50	50	50	HG518500	7/7
		R: TGAGAGATGTTCCTGTTCTG
TTG-11	(CAA)₁₀	F: GTTTTGTAGCTCTGTTGGAC	251	50	50	50	50	50	50	50	HG518501	7/7
		R: CCATATGACCTAGCTTCCCA
TTG-49	(AGG)₈	F: GAGATGACAGAGGCTGCTG	224	55	55	55	55	55	55	55	HG518502	7/7
		R: GCTAGTGGGTGCACTAGGT
TTG-54	(GA)₁₅(GAA)₂	F: CTCGTGCTAACCCTTGATC	222	50	50	50	50	50	50	50	HG518503	7/7
		R: GGCTACCTTAGGATGTGTGCA

Note: TW = K. davidiana var. formosana; cy = K. fortunei var. cyclolepis; pu = K. pubescens; ca = K. davidiana var. calcarea; cp = K. davidiana var. chien-peii; fo = K. fortune; ev = K. evelyniana; F = the forward primer; R = the reverse primer; Ta = optimized annealing temperature.

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ISSN: 1756-0500

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