Figure 1

TrackArt SIM. Simulations in Simple and Switch mode. A-B. A single trajectory of a particle diffusing with D = 2 μm²/s, with simulated localization uncertainty σ = 0 nm and σ = 200 nm respectively. C. Simple mode simulation of four particles diffusing with D ₁  = 2 and D ₂  = 0.2 μm²/s, fraction = 50%. D. Switch mode simulation of four particles diffusing with D ₁  = 2 and D ₂  = 0.2 μm²/s. Constant rates for switch between states are equal to 0.5. For all simulations (A-D) N = 1000 steps per trajectory, Δt = 10 ms.