DNA methylation signatures in cord blood associated with maternal gestational weight gain: results from the ALSPAC cohort

Table 3 Validation study: associations of maternal pre-pregnancy BMI and gestational weight gain (GWG) with MMP7 CpG sites methylation levels in newborn cord blood DNA

	CpG1		CpG2		CpG3 (E59)		Mean of 3 CpGs
	Coef^a(se)	p value	Coef^a(se)	p value	Coef^a(se)	p value	Coef^a(se)	p value
Pre-pregnancy BMI (n = 87)	0.80 (0.70)	0.250	1.15 (0.78)	0.143	1.22 (0.82)	0.139	1.06 (0.75)	0.158
GWG 0 to 18 wks (n = 84)	0.80 (1.99)	0.686	1.52 (2.27)	0.505	2.44 (2.36)	0.302	1.59 (2.15)	0.461
GWG 19 to 28 wks (n = 84)	0.96 (1.73)	0.580	2.89 (1.95)	0.141	2.83 (2.04)	0.167	2.23 (1.86)	0.232
GWG 29 wks. onwards (n = 84)	2.38 (1.57)	0.133	3.25 (1.79)	0.071	3.90 (1.85)	0.037	3.17 (1.69)	0.062
GWG IoM categories (n = 78)
Recommended	0 [Ref.]		0 [Ref.]		0 [Ref.]		0 [Ref.]
Less than recommended	2.03 (1.74)	0.246	0.18 (2.00)	0.930	0.38 (2.09)	0.853	0.86 (1.90)	0.649
More than recommended	3.35 (1.79)	0.063	3.13 (2.06)	0.131	3.81 (2.16)	0.080	3.43 (1.96)	0.082

^aRegression coefficient using linear mixed models. For pre-pregnancy BMI (kg/m2) estimated changes per 1 SD change and for GWG estimated changes per additional 400 g/week.
All models adjusted for child’s sex and maternal age and the inclusion of a PCR plate random batch effect.

ISSN: 1756-0500