Skip to main content
Figure 2 | BMC Research Notes

Figure 2

From: Complete steady-state rate equation for DNA ligase and its use for measuring product kinetic parameters of NAD+-dependent DNA ligase from Haemophilus influenzae

Figure 2

Global non-linear least-squares fit of data from NMN product inhibition of H. influenzae DNA ligase. The data were fit to the Bi Ter Ping Pong Uni-Uni Uni-Bi steady-state rate equation with product P present: V = V max [A][B]/(K mB [A] + K mA [B](1 + [B]/K I ) + [A][B] + (K ia K mB /K ip )[P] + (K mB /K ip )[A][P] + {K ia K mB /(K ip K I )}[B][P]). Each data point represents a single measurement. The 60 nM DNA data at 0 mM AMP were omitted due to poor quality.

Back to article page