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Figure 3 | BMC Research Notes

Figure 3

From: Complete steady-state rate equation for DNA ligase and its use for measuring product kinetic parameters of NAD+-dependent DNA ligase from Haemophilus influenzae

Figure 3

Global non-linear least-squares fit of data from AMP product inhibition of H. influenzae DNA ligase. The data were fit to the Bi Ter Ping Pong Uni-Uni Uni-Bi steady-state rate equation with product R present: V = V max [A][B]/(K mB [A] + K mA [B](1 + [B]/K I  + [R]/K ir ) + [A][B]) Each data point represents a single measurement.

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