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Table 1 Kinetic parameters obtained by nonlinear least-squares global fitting of data from Figures 2 and 3

From: Complete steady-state rate equation for DNA ligase and its use for measuring product kinetic parameters of NAD+-dependent DNA ligase from Haemophilus influenzae

Parameter Value from NMN study Value from AMP study
Vmax 150 ± 15 nmol/min/mg 169 ± 13 nmol/min/mg
KmA 650 ± 80 nM 410 ± 50 nM
KmB 62 ± 9 nM 85 ± 9 nM
Ki 66 ± 14 nM 32 ± 8 nM
Kia 610 ± 120 nM ---
Kip 3.3 ± 0.4 μM ---
Kir --- 390 ± 60 μM
  1. KmA and KmB are the Michaelis constants for NAD+ and nDNA, respectively. Ki is the dissociation constant for nDNA with unadenylylated DNA ligase (i.e. substrate inhibition constant for nDNA). Kia is the dissociation constant for NAD+ with unadenylylated DNA ligase. Kip is the dissocation constant for NMN. Kir is the dissociation constant for AMP. Standard errors of best-fit values are shown.