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Table 1 Eight candidate reference genes assessed for gene expression normalisation in Olea europaea and amplicon characteristics

From: Validation of reference genes for gene expression analysis in olive (Olea europaea) mesocarp tissue by quantitative real-time RT-PCR

Gene name

Accession number/cluster ID*

Primer sequence (5' to 3')

Amplicon length (bp)

Annealing temperature** (°C)

PCR efficiency value***

Standard error (SE)

R2****

60S RBP L18-3

[Oleadatabase:Cluster ID-OLEEUCl011221] contig 2

F: GTAAGAGCAAGAAGACCAAG

101

55

1.984

0.014

0.978

R: GCTTCCAGTTCTCCTCAC

PP2A

[Oleadatabase:Cluster ID-OLEEUCl010038] contig 1

F: AGATCGGTGAAATACTTCCACACG

189

56

2.038

0.020

0.969

R: TCGTGGATACTACTCAGTGGAGACTG

PTB

[Oleadatabase:Cluster ID-OLEEUCl031691] contig 1

F: CTTCTCCGAAATAAACCAGAT

156

56

2.243

0.057

0.855

R: GGTGTCAGCTCCAGTTGTAA

TUBA

[Oleadatabase:Cluster ID-OLEEUCl051890] contig 1

F: AGAACACCTCAGCAACAC

100

51

1.704

0.069

0.870

R: AACTACCAGCCACCAACT

TIP2

[Oleadatabase:Cluster ID-OLEEUCl011159] contig 2

F: ACTTGTTGTAAGCAATGG

104

51

2.093

0.081

0.935

R: TGATTCATTAAGCGTTGG

OUB2

[GenBank:AF429430.1]

F: AATGAAGTCTGTGTGTCCTTTGG

150

51

1.513

0.054

0.890

R: AAGGGAAATCCCATCAACG

GAPDH

[GenBank:EF506530.1]

F: ACAGCTCCTGGTAAGGGTGA

210

56

2.111

0.018

0.971

R: GGCTTGCGTCAAGAAGTCTC

EF1-alpha

[GenBank:XM_002527974.1]

F: GAATGGTGATGCTGGTTTCG

191

56

1.908

0.005

0.996

R: CCCTTCTTGGCAGCAGACTTG

  1. *Cluster ID obtained from the Olea database[26].
  2. **Annealing temperature used in PCR experiments.
  3. ***Measure of the PCR amplification efficiency calculated from calibration curve derived from prepared standards.
  4. ****correlation coefficient (R2) for the calibration curve.
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BMC Research Notes

ISSN: 1756-0500

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