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Table 1 Ten eukaryotic primers used in complement with those reported in a previous study[2]

From: Gut microeukaryotes during anorexia nervosa: a case report

Taxon

Primer

Target

PCR product size (bp)

Annealing temperature (°C)

Reference

Helminthes

NC1/NC2

18S rRNA gene

310-410

55

[3]

 

TDR5/TDR3

 

1700

57

[3]

Blastocystis spp.

SF/SR

18S rRNA gene

600

55

[4]

Plasmodium spp.

PLAS1/PLAS2

Cytochrome b gene

709

55

[5]

 

PLAS3/PLAS4

 

709

55

[5]

Plant

rD5-ITS2/rb1-ITS2f

ITS-2 gene

350

59

[6]

Arthropoda

ZBJ-ArtF1c/ZBJ-ArtR2c

Mitochondrial coxI gene

157

56-57

[7]

 

CB3/CB4

Cytochrome b gene

410

46

[8]

Leishmania spp.

LeF/LeR

18S rRNA gene

330

65

[9]

 

LEI70R/LEI70L

  

65

[9]