Table 1 Experimental design showing 13 rehydration, light, and temperature conditions for three specimens of Lobaria pulmonaria
From: Rehydration conditions for isolation of high quality RNA from the lichen Lobaria pulmonaria
Treatment | Rehydration with water | Temp (°C) | Light | Rehydration time (hours) | No. replicates for VM5172 | No. replicates for VM5174 | No. replicates for VM5190 |
|---|---|---|---|---|---|---|---|
1 | no | 20 | yes | 0 | 3 | 3 | 3 |
2 | yes | 20 | yes | 0.5 | 3 | 3 | 3 |
3 | yes | 20 | yes | 1 | 3 | 3 | 3 |
4 | yes | 20 | yes | 2 | 3 | 3 | 3 |
5 | yes | 20 | yes | 4 | 3 | 3 | 3 |
6 | yes | 20 | yes | 24 | 3 | 3 | 3 |
7 | yes | 20 | no | 1 | 3 | 3 | 3 |
8 | yes | 20 | no | 4 | 3 | 3 | 3 |
9 | yes | 32 | yes | 1 | 3 | 3 | 3 |
10 | yes | 32 | yes | 4 | 3 | 3 | 3 |
11 | yes | 15 | yes | 1 | 3 | 3 | 3 |
12 | yes | 15 | yes | 4 | 3 | 3 | 3 |
13 | yes | 15 | yes | 24 | 3 | 3 | 3 |