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Table 2 Reference and target gene efficiency determination and amplicon melting points, r 2 values are regression coefficients for the curves

From: Identification of appropriate reference genes for qPCR studies in Staphylococcus pseudintermedius and preliminary assessment of icaA gene expression in biofilm-embedded bacteria

Candidate reference genes

Slope of the curve

r2on the slope

PCR efficiency

Melting temperature (°C)

proC

−3.78

0.997

1.84

80.0

gyrB

−3.85

0.998

1.82

79.5

rplD

−3.86

0.998

1.82

75.5

rho

−3.68

0.997

1.87

81.0

rpoA

−3.89

0.996

1.81

76.5

ftsZ

−3.85

0.998

1.82

79.0

recA

−3.83

0.998

1.82

81.0

sodA

−3.97

0.996

1.79

79.5

Target gene

    

icaA

−3.92

0.995

1.80

80.5