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Table 5 Candidate reference and target genes, primers and amplicon sizes

From: Identification of appropriate reference genes for qPCR studies in Staphylococcus pseudintermedius and preliminary assessment of icaA gene expression in biofilm-embedded bacteria

Candidate reference genes

Function

Primer sequence (5’-3’)

Expected size (bp)

proC

Pyrrolidine-5-carboxylate reductase

proC-F gccgaatacaaatgcgcacg

180

proC-R aaaaatgcagggccacttcc

 

gyrB

DNA gyrase B subunit

gyrB-F gcgtccgttgattgaagcg

240

gyrB-R aacgtcacttgcaacatcgc

 

rplD

50S ribosomal protein L4

rplD-F gcctaagaaaatgcgtcg

237

rplD-R ccttctggtgttgtgattg

 

rho

Transcription termination factor Rho

rho-F cacgtaaaagttgctgaattg

215

rho-R cctgcttcgatatttctgg

 

rpoA

DNA-directed RNA polymerase sigma factor

rpoA-F ctatcatcattaccaggtgc

231

rpoA-R caaaatttcaacatcactgtc

 

ftsZ

Cell division protein ftsZ

ftsZ-F gtccattcagtttcgaagg

254

ftsZ-R catgattgttttaacgtcagc

 

recA

Recombinase A

recA-F gcattaggtgtagatattgataac

228

recA-R ggctgcagaaagtttacgc

 

sodA

Superoxide dismutase

sodA-F cgcaaacttagacagcgtacc

227

sodA-R caacaagccaagcccaacc

 

Target Gene

   

icaA

N-acetylglucosaminyl-transferase

icaA-F ttgcccaccttgtgcccacc

178

  

icaA-R tgaggctgtagggcgttggga

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