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Table 2 Population genetic parameters of microsatellite loci of Garra barreimiae and cross-species amplification results

From: Characterization of 19 new microsatellite loci for the Omani barb Garra barreimiae from 454 sequences

Locus name

N MF

N WF

Size (bp)

NA

HeMF

HoMF

Oosterhout MF

HW p-value MF

HeWF

HoWF

Oosterhout WF

HW p-value WF

FST

CA

QLIMC

20

24

88 - 92

3

0.00

0.00

0

NV

19.42

20.83

−0.108

0

0.059

22

7GHL

20

24

166 - 250

19

89.10

90.00

−0.022

0.571

93.09

95.83

−0.027

0.777

0.025*

11

PH8AC

20

24

126 - 188

15

86.03

95.00

−0.076

0.634

91.58

75.00

0.082

0.995

0.020*

21

UHPEC

20

24

89 - 101

7

35.26

30.00

0.107

0.872

54.43

62.50

−0.087

0.437

0.071*

20

JQSOC

20

24

147 - 169

7

74.49

75.00

−0.021

0.092

75.89

62.50

0.077

0.966

0.160**

20

DL6X

20

24

112 - 126

8

60.00

65.00

−0.069

0.250

59.75

70.83

−0.120

0.273

0.156**

19

FYP9C

20

24

169 - 235

20

78.46

90.00

−0.096

0.477

95.04

87.50

0.031

0.793

0.068**

22

HOLNC

20

24

191 - 233

7

59.49

65.00

−0.067

0.128

39.89

41.67

−0.033

0.419

0.195**

22

JMLRC

20

24

93 - 99

4

57.44

65.00

−0.064

0.360

71.37

70.83

−0.007

0.818

0.120**

20

CJHGC

20

24

198 - 215

7

54.23

35.00

0.172

0.968

77.66

75.00

−0.002

0.588

0.194**

21

3Z71C

20

24

123 - 127

2

29.62

35.00

−0.194

0.000

0.00

0.00

0

NV

0.170*

22

9XNCC

20

24

101 - 112

6

71.67

70.00

−0.012

0.594

74.20

70.83

0.007

0.589

0.061*

22

3 N43

20

24

182 - 184

2

0.00

0.00

0

NV

28.37

33.33

−0.184

0

0.135*

22

1EHEC

20

24

106 - 112

4

68.08

80.00

−0.106

0.000

63.03

62.50

0.004

0.068

0.176**

22

88CMC

20

24

190 - 198

6

40.90

45.00

−0.069

0.000

73.40

54.17

0.120

0.995

0.216**

21

I6G2C

20

24

115 - 137

8

58.21

60.00

−0.012

0.684

71.45

62.50

0.048

0.781

0.282**

22

3ROZ

20

24

262 - 276

5

14.23

5.00

0.172

0.923

56.34

4.55

0.386

1.000

0.172*

0

CB75

20

24

171 - 199

7

51.41

45.00

0.067

0.767

68.17

75.00

−0.069

0.074

0.005

2

2PUMC

20

24

152 - 176

8

38.33

45.00

−0.240

0.000

77.66

87.50

−0.082

0.212

0.121**

22

  1. N number of individuals analysed, Ta (°C) annealing temperature, NA number of alleles, MF Misfat Al Abriyeen, WF Wadi Al Falahi, He expected heterozygosity in%, Ho observed heterozygosity in%, null allele estimates (Oosterhout values), HW p-value probability for deviation from Hardy-Weinberg equilibrium, FST values (*indicates significant values at p < 0.05 and **at p < 0.001); loci marked with Cwere positive and polymorphic in Garra rufa; CA number of individuals positive in cross-species amplification (tested in 22 individuals of G. rufa).