Figure 7

Analysis of cell cycle phases of ADSCs from males and females. Results represent means ± S.D. from three independent experiments of each experimental group (days and sexes) in triplicate, obtained by flow cytometry analysis. A: The proportion of ADSCs from C57BL/6 J females and males 30 days residing in sub-haploid, G0/G1, S and G2/M are indicated; B: The proportion of ADSCs from C57BL/6 J females and males with 120 days in sub-haploid phase, G0/G1, S and G2/M was indicated. Statistical differences were obtained by analysis of variance ANOVA and Tukey-Kramer multiple-comparisons test. Statistical significance (p-value) *p < 0.05. C, D, E, and F: Cell cycle analysis of flow cytometric DNA histograms. C: Histogram shows the proportion of ADSCs from C57BL/6 J females 30 days in sub-haploid phase, G0/G1, S and G2/M cell cycle phases; D: Histogram shows the proportion of ADSCs from C57BL/6 J males 30 days in sub-haploid phase, G0/G1, S and G2/M cell cycle phases; E: Histogram shows the proportion of ADSCs from C57BL/6 J females 120 days in sub-haploid phase, G0/G1, S and G2/M cell cycle phases; F: Histogram shows the proportion of ADSCs from C57BL/6 J males 120 days in sub-haploid phase, G0/G1, and G2/M cell cycle phases. The histograms were obtained by ModFit 3.2 software.