Table 1 The most basic requirements for evaluating protein quality

In-deep protein biophysical characterization needs specific expertise and specialized equipment, but any biology lab should be able to assess the produced proteins using to at least two complementary techniques:

1.

PAGE-SDS provides multiple information regarding the quality of the protein such as the presence of degradation products as well as the absence of protein contamination.

2.

Analytical size exclusion chromatography (SEC) [18] provides information regarding the correct oligomeric structure of the protein and the absence of soluble aggregates that can cause non-specific results in downstream experiments.